Jamestown is served by the Burlington Northern Santa Fe Railroad which does not provide passenger service but does provide freight service. A rail spur is currently being installed at the Industrial Park east of the city.
Amtrak does run through the state and travels east and west across the state starting at Fargo and angling north to Devils Lake, Minot and Williston.
Jamestown is the intersecting point of Highways Interstate 94 and U.S. Highway 281. Interstate 94 runs east and west across the state and Highway 281 runs north and south, starting at the Canadian border and continues south to Brownsville, Texas.
Jamestown is served by two taxi companies, Jamestown Taxi Service and Last Leg Taxi.
James River Public Transit provides Dial-A-Ride services for people of all ages. Five to six buses serve the City of Jamestown on a daily basis. Phone (701) 252-7888 for rides.